Characterization of Hemagglutinin Gene Fragment of H9N2 Avian Influenza Virus Isolated from Environmental Live Bird Market in the Greater Jakarta Area

Virus H9N2 has become the most common subtype of low pathogenic avian influenza (LPAI) in poultry and shows ability to infect humans. One important factors triggering virus ​​pandemic is live bird market (LBM). The acts as an internal gene donor other subtypes, such H5N1, H5N2, H7N9, H10N8, This study was conducted detect presence molecular characteristics hemagglutinin fragment at cleavage site, receptor binding antigenic glycosylation site positions AI isolated from LBM environment. used Disease Investigation Center Subang isolates environmental samples Greater Jakarta area (DKI Jakarta, Bogor, Depok, Tangerang, Bekasi) 2019. Based on detection using RT-PCR RT-qPCR, it found that detected. indicates potential be source spread ​​H9N2 virus. pattern amino acid PSRSSR↓GLF research sample belongs AI. substitution acids increases specificity non-human host recognition. with NCS motif position 295-297, close receptor-binding site. genetic analysis phylogenetic topography, included CVI (China, Vietnam, Indonesia) lineage subclade H9.4.2.5.